development of a method for dna extraction from safflower seed oil leaves
Safflower Seed Extract Attenuates the Development of
Furthermore, safflower seed extract suppressed OA development in the DMM-induced OA model. The action mechanism of safflower seed extract and single compounds involves the regulation of NF-κB transcription through the IκB-p65 signaling pathway. IL-1β is known to modulate the expression of catabolic factors in mouse chondrocytes [40,41
Appropriate statistical methods for analysis of safflower
Also, its oil has a high quality and nutritional value, due to high proportion of polyunsaturated fatty acids (>80%) (Usha Kiran et al., 2017). However, there is evidence from the literature that safflower seed yield and seed oil content remain in general low (Ali et al., 2019). Thus, there is a need to breed and improve the performance of
Agrobacterium-mediated transformation of safflower and the
Southern-blot analysis of nine independent lines of transgenic safflower. Genomic DNA was prepared from the leaves of independent T 0 lines from two varieties; S317 or WT. gDNA was digested with the restriction enzyme HindIII and probed using a labeled GFP sequence. Lane 1-5 transgenic plants from S-317 and lane 7-10 transgenic plants from WT.
Sunflower Mustard Extraction of safflower seed oil by supercritical CO 2
Additionally, the quality of safflower seed oil obtained by supercritical CO2 extraction Mathematical model is superior to that of oil obtained by traditional methods. It is noted that a new method of changing flow Scale up rate was proposed to improve the process efficiency and proved to be valuable by experiment.
Extraction of safflower seed oil by supercritical CO2
Additionally, the quality of safflower seed oil obtained by supercritical CO 2 extraction is superior to that of oil obtained by traditional methods. It is noted that a new method of changing flow rate was proposed to improve the process efficiency and proved to be valuable by experiment.
Frontiers | Protein Quantity and Quality of Safflower Seed
It is used as a source of dye, medicines and food. It is cultivated as a source of oil and protein. It contains 34% oil and 22–24% protein and its seeds are rich source of natural antioxidant (tocopherol). The seed meal of safflower seeds after oil extraction is utilizd as a cattle feed and organic fertilizer.
Development of SCAR markers linked to male sterility and very
Ten fully expanded leaves were cut from each of the 162 F 2 plants and frozen at −80°C. The leaf tissue was lyophilized and ground to a fine powder in a laboratory mill. DNA was isolated from ground leaf tissue from each F 2 plant using a modified version of the protocol described by Rogers and Bendich . DNA was also isolated from five plants of the CL1 and CR-142 parents.
A review of fatty acids and genetic- World Journal
Safflower showed great changes in the contents of seed oil and in the fatty acid compositions during seed development. Sims et al [21] . reported that, in developing safflower seeds, the oleic acid concentration increased slowly during the first 30 days after fertilization and then in some cases appeared to level off with approaching maturity.
Sunflower Mustard Nonsense-mediated mRNA degradation of- ResearchGate
Safflower seed is a source of a-tocopherol (determined by high performance liquid chromatography method with fluorescence detection) and its amount ranged from 358.8 to 461.8 mg/L of oil.
Seed yield and oil quality of sunflower, safflower, and
Safflower (Carthamus tinctorius L.), whose origin is largely unknown (Chapman and Burke, 2007), is a major oilseed crop that yields 32–40% seed oil (Soliman et al., 2011). Safflower oil is utilized in many industries for edible and dying purposes.
Sunflower Mustard Phytochemistry, pharmacology and- ResearchGate
Safflower seeds provide an oil rich in mono and polyunsaturated fatty acids. Its adaptation to drought and high temperatures makes it an alternative for the development of oleaginous crops in semi
Sequence variations and expression analysis of FAD2 gene
The seedling leaves were harvested after a week, chilled in liquid nitrogen and stored at − 70 °C until DNA extraction. The developmental seeds at 2, 6, 10, 14, 18, 22, 26 DAF were harvested for PI and PI, which were the three different representative LA-type materials selected among 15 genetic stable safflower materials, chilled in liquid nitrogen and stored − 70 °C until fatty acid and RNA extraction.
Genome-wide identification and- Scientific Reports
Genome-wide identification of CtbZIP TFs. Through in silico analyses (detailed in methods), a total of 52 members in safflower bZIP gene family were identified. Based on the splicing results of
A case study on the genetic origin of the high- Frontiers in
The safflower (Carthamus tinctorius L.) is considered a strongly domesticated species with a long history of cultivation. The hybridization of safflower with its wild relatives has played an important role in the evolution of cultivars and is of particular interest with regards to their production of high quality edible oils. Original safflower varieties were all rich in linoleic acid, while
Antioxidant and Antimicrobial Potentials of Seed Oil from
Scavenging activity of Safflower seed oil against DPPH and ABTS free radicals was evaluated as described . 180 μL of 0.1 mM DPPH solution was mixed with 20 μL of safflower oil. The mixture was shaken vigorously then left to incubate at room temperature in the dark for 30 min.
Extraction, Structures, Bioactivities and Structure-Function
Interestingly, traditional hot water extraction remains the method with the best extraction efficiency to date. A schematic representation of the extraction methods of PBPC is shown in Figure 2. In these approaches, the walls of safflower bee pollen are broken and the material is refluxed with petroleum ether (2 h) and 95% ethanol (two times) successively.
Cosmetics | Free Full-Text | Phenol Content and Antioxidant
This method consists of oil extraction with an organic solvent (cyclohexane) on 20 g of solid matrix (seed crushed) for 6 h with a ratio of 1:10 w:v. The solvent containing oil was removed using a rotary evaporator at a temperature of 45 °C.
TRANSFORMATION USING AGROBACTERiUM TUMEFACIENS
The oil extracted from safflower seed is desirable in the paint and varnish industries because of its non-yellowing properties. Research conducted by the US Department of Energy has shown that industrial grade safflower oil has great promise as a viable alternative to the use of fossil fuels (Bergman and Flynn, 1987).
A large and functionally diverse family- BMC Plant Biology
The genomic DNA of safflower genotype SU was isolated from fully expanded leaves using CTAB buffer following the method described by Paterson et al. (1993). Further purification was carried out using CsCl gradient as previously described .
Sunflower Mustard Agrobacterium -mediated transformation of safflower and
Safflower is accesses sub-soil water reserves [6]. grown for its edible oil (high oleic and high linoleic vari- Despite commercial activities based on the genetic eties), high-protein seed cake, animal meal, bird seed modification of safflower, there is no method available and for traditional medicine [1,2].
Agrobacterium-mediated transformation of safflower and the
GFP was detected in extracts of safflower leaves using an earlier reported protocol . Briefly, 100 mg fresh safflower leaf was crushed in 200 μL laemelli buffer (2% SDS, 100 mM Tris-base, pH 7.2, 20% glycerol, 60 mM dithiothreol; 0.02% bromophenol blue) and heated at 95°C for 5 min before centrifuging at room temperature at g for 5 min. 10 μL of supernatant was loaded onto a 12.5% polyacrylamide gel (12.5% PAGE-Sprint Buffer System; Amresco laboratory supplies) and resolved using
Comprehensive analysis of volatile- Wiley Online Library
2 MATERIALS AND METHODS 2.1 Samples. Safflower seeds from the three most popular cultivars (Tacheng HH‐4, Bole HH‐1, and Changji HH‐2) were harvested in early September 2017 from the Xinjiang Autonomous Region, China. The safflower seeds were cold‐pressed by oil press (ZY‐22A) at temperature of 45°C.
Molecular diversity assessment of a world collection of
The young leaves were used for DNA extraction according to modified CTAB protocol described by (Murray and Thompson 1980). DNA concentration was measured with a Nano Drop, spectrophotometer (Nano Drop Technologies Inc Wilmington, DE, USA), and DNA was diluted to a working concentration of 50 ng/µl.
Identification and Evaluation of Reference Genes for- PLOS
Safflower (Carthamus tinctorius L.) has received a significant amount of attention as a medicinal plant and oilseed crop. Gene expression studies provide a theoretical molecular biology foundation for improving new traits and developing new cultivars. Real-time quantitative PCR (RT-qPCR) has become a crucial approach for gene expression analysis. In addition, appropriate reference genes (RGs
Development of SCAR markers linked to male sterility and very
Practically no molecular tools have been developed so far for safflower (Carthamus tinctorius L.) breeding. The objective of the present research was to develop molecular markers for the closely linked genes Li, controlling very high linoleic acid content, and Ms, controlling nuclear male sterility (NMS). A mapping population of 162 individuals was developed from the NMS line CL1 (64–79%
Antioxidants | Free Full-Text | Herbal Additives
The aim of the study was to examine combinations of base oils and herbal additives with a view to obtaining macerates with improved health benefits. Base oils were cold-pressed from the seeds of black cumin, borage, evening primrose, safflower, walnut, common hazel, and oilseed rape, as well as the flesh of sea-buckthorn fruits. They were then supplemented with herbs, including basil, thyme
Mobile genomic element diversity in world collection- PLOS
Safflower (Carthamus tinctorius L.) is a multipurpose crop of dry land yielding very high quality of edible oil. Present study was aimed to investigate the genetic diversity and population structure of 131 safflower accessions originating from 28 different countries using 13 iPBS-retrotransposon markers. A total of 295 iPBS bands were observed among which 275 (93.22%) were found polymorphic
Seed-specific RNAi in safflower generates a superhigh- Wiley
SHO safflower oil is the result of seed-specific hairpin-based RNA interference of two safflower lipid biosynthetic genes, FAD2.2 and FATB, producing seed oil containing less than 1.5% polyunsaturates and only 4% saturates but with no impact on lipid profiles of leaves and roots. Transgenic SHO events were compared to non-GM safflower in
AgBioView Newsletter on Agricultural Biotechnology
In the safflower seed case, the Apo A1 gene is only expressed in the seed. The technology, which can be used in any oil seed, has been further developed so that the protein attaches itself to oil bodies in the seed making the recovery of the protein much easier as the oil floats out of the solution when the seeds are crushed to extract the protein.